Review





Similar Products

s mutans ua159 wt genome sequence reference strain atcc ∆ rm dpnmab mutant  (ATCC)
94
ATCC s mutans ua159 wt genome sequence reference strain atcc ∆ rm dpnmab mutant
S Mutans Ua159 Wt Genome Sequence Reference Strain Atcc ∆ Rm Dpnmab Mutant, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mutant+dna+sequence/pmc12542736__aem%2E01094-25-s0001-8-0-8?v=ATCC
Average 94 stars, based on 1 article reviews
s mutans ua159 wt genome sequence reference strain atcc ∆ rm dpnmab mutant - by Bioz Stars, 2026-07
94/100 stars
  Buy from Supplier
mutant il2 dna sequence  (Azenta)
86
Azenta mutant il2 dna sequence
Mutant Il2 Dna Sequence, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mutant+dna+sequence/us12564632-546-9-29?v=Azenta
Average 86 stars, based on 1 article reviews
mutant il2 dna sequence - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier
mutant il 10 dna sequence  (Azenta)
86
Azenta mutant il 10 dna sequence
Mutant Il 10 Dna Sequence, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mutant+dna+sequence/us12564632-906-9-29?v=Azenta
Average 86 stars, based on 1 article reviews
mutant il 10 dna sequence - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier
mutant dna sequences  (Sangon Biotech)
90
Sangon Biotech mutant dna sequences
Mutant Dna Sequences, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mutant+dna+sequence/pm40167214-41-4-11?v=Sangon+Biotech
Average 90 stars, based on 1 article reviews
mutant dna sequences - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
dna sequences for enst00000198536.7 (wildtype) and enst00000198536.7 containing rs2405442:t>c (mutant)  (GenScript corporation)
90
GenScript corporation dna sequences for enst00000198536.7 (wildtype) and enst00000198536.7 containing rs2405442:t>c (mutant)
Dna Sequences For Enst00000198536.7 (Wildtype) And Enst00000198536.7 Containing Rs2405442:T>C (Mutant), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mutant+dna+sequence/pm40149715-59-6-13?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
dna sequences for enst00000198536.7 (wildtype) and enst00000198536.7 containing rs2405442:t>c (mutant) - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
recombinant dna mutant cdh1 sequences addgene  (Addgene inc)
94
Addgene inc recombinant dna mutant cdh1 sequences addgene
Figure 1. Disruption of apical-basal polarity by dominant-negative <t>CDH1</t> mutants in the Matrigel overlay culture system or by Cdc42 knockout in the developing mouse pancreas alters beta cell specification (A) Design of dominant-negative CDH1 mutants with deletions in the extracellular domain (CDH1DE) or the p120ctn-binding site (CDH1DP). mCh, internal ribosome entry site (IRES)-coupled mCh (red fluorescent protein) reporter. (B) Confocal images of S5 (day 13) differentiated cells (± 72 h Dox treatment). EZR, EZRIN, green; BCAT, beta-catenin, gray; mCh, mCherry, red. Scale bar, 50 mm. (C) Flow cytometry quantification of INS single-positive beta cells and GCG positive alpha cells in mCherry+ and mCherry subpopulations after Dox treatment (S4–S6). CDH1DE and CDH1DP mutations led to the same degree of reduction in the INS+GCG/GCG+ ratio in mCherry+ mutant cells compared with mCherry
Recombinant Dna Mutant Cdh1 Sequences Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mutant+dna+sequence/pm40056911-544-174-179?v=Addgene+inc
Average 94 stars, based on 1 article reviews
recombinant dna mutant cdh1 sequences addgene - by Bioz Stars, 2026-07
94/100 stars
  Buy from Supplier
dna fragments of wildtype or codon mutant pycard sequence and linker sequence (gsgsg)  (Twist Bioscience)
90
Twist Bioscience dna fragments of wildtype or codon mutant pycard sequence and linker sequence (gsgsg)
Figure 1. Disruption of apical-basal polarity by dominant-negative <t>CDH1</t> mutants in the Matrigel overlay culture system or by Cdc42 knockout in the developing mouse pancreas alters beta cell specification (A) Design of dominant-negative CDH1 mutants with deletions in the extracellular domain (CDH1DE) or the p120ctn-binding site (CDH1DP). mCh, internal ribosome entry site (IRES)-coupled mCh (red fluorescent protein) reporter. (B) Confocal images of S5 (day 13) differentiated cells (± 72 h Dox treatment). EZR, EZRIN, green; BCAT, beta-catenin, gray; mCh, mCherry, red. Scale bar, 50 mm. (C) Flow cytometry quantification of INS single-positive beta cells and GCG positive alpha cells in mCherry+ and mCherry subpopulations after Dox treatment (S4–S6). CDH1DE and CDH1DP mutations led to the same degree of reduction in the INS+GCG/GCG+ ratio in mCherry+ mutant cells compared with mCherry
Dna Fragments Of Wildtype Or Codon Mutant Pycard Sequence And Linker Sequence (Gsgsg), supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mutant+dna+sequence/pmc11323107-372-4-22?v=Twist+Bioscience
Average 90 stars, based on 1 article reviews
dna fragments of wildtype or codon mutant pycard sequence and linker sequence (gsgsg) - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
sequencing of mutant strains and dna constructs  (Macrogen)
90
Macrogen sequencing of mutant strains and dna constructs
Figure 1. Disruption of apical-basal polarity by dominant-negative <t>CDH1</t> mutants in the Matrigel overlay culture system or by Cdc42 knockout in the developing mouse pancreas alters beta cell specification (A) Design of dominant-negative CDH1 mutants with deletions in the extracellular domain (CDH1DE) or the p120ctn-binding site (CDH1DP). mCh, internal ribosome entry site (IRES)-coupled mCh (red fluorescent protein) reporter. (B) Confocal images of S5 (day 13) differentiated cells (± 72 h Dox treatment). EZR, EZRIN, green; BCAT, beta-catenin, gray; mCh, mCherry, red. Scale bar, 50 mm. (C) Flow cytometry quantification of INS single-positive beta cells and GCG positive alpha cells in mCherry+ and mCherry subpopulations after Dox treatment (S4–S6). CDH1DE and CDH1DP mutations led to the same degree of reduction in the INS+GCG/GCG+ ratio in mCherry+ mutant cells compared with mCherry
Sequencing Of Mutant Strains And Dna Constructs, supplied by Macrogen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mutant+dna+sequence/pm38527561-63-0-10?v=Macrogen
Average 90 stars, based on 1 article reviews
sequencing of mutant strains and dna constructs - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
dna sequence for the m1199l mutant peptide, dadm1199l  (GenScript corporation)
90
GenScript corporation dna sequence for the m1199l mutant peptide, dadm1199l
Figure 1. Disruption of apical-basal polarity by dominant-negative <t>CDH1</t> mutants in the Matrigel overlay culture system or by Cdc42 knockout in the developing mouse pancreas alters beta cell specification (A) Design of dominant-negative CDH1 mutants with deletions in the extracellular domain (CDH1DE) or the p120ctn-binding site (CDH1DP). mCh, internal ribosome entry site (IRES)-coupled mCh (red fluorescent protein) reporter. (B) Confocal images of S5 (day 13) differentiated cells (± 72 h Dox treatment). EZR, EZRIN, green; BCAT, beta-catenin, gray; mCh, mCherry, red. Scale bar, 50 mm. (C) Flow cytometry quantification of INS single-positive beta cells and GCG positive alpha cells in mCherry+ and mCherry subpopulations after Dox treatment (S4–S6). CDH1DE and CDH1DP mutations led to the same degree of reduction in the INS+GCG/GCG+ ratio in mCherry+ mutant cells compared with mCherry
Dna Sequence For The M1199l Mutant Peptide, Dadm1199l, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mutant+dna+sequence/pm37832872-205-8-12?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
dna sequence for the m1199l mutant peptide, dadm1199l - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
pif7-2 (syngenta collection of sequenced t-dna insertional mutants, line 622)  (Syngenta)
90
Syngenta pif7-2 (syngenta collection of sequenced t-dna insertional mutants, line 622)
Figure 1. Disruption of apical-basal polarity by dominant-negative <t>CDH1</t> mutants in the Matrigel overlay culture system or by Cdc42 knockout in the developing mouse pancreas alters beta cell specification (A) Design of dominant-negative CDH1 mutants with deletions in the extracellular domain (CDH1DE) or the p120ctn-binding site (CDH1DP). mCh, internal ribosome entry site (IRES)-coupled mCh (red fluorescent protein) reporter. (B) Confocal images of S5 (day 13) differentiated cells (± 72 h Dox treatment). EZR, EZRIN, green; BCAT, beta-catenin, gray; mCh, mCherry, red. Scale bar, 50 mm. (C) Flow cytometry quantification of INS single-positive beta cells and GCG positive alpha cells in mCherry+ and mCherry subpopulations after Dox treatment (S4–S6). CDH1DE and CDH1DP mutations led to the same degree of reduction in the INS+GCG/GCG+ ratio in mCherry+ mutant cells compared with mCherry
Pif7 2 (Syngenta Collection Of Sequenced T Dna Insertional Mutants, Line 622), supplied by Syngenta, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mutant+dna+sequence/pmc10055326-123-40-33?v=Syngenta
Average 90 stars, based on 1 article reviews
pif7-2 (syngenta collection of sequenced t-dna insertional mutants, line 622) - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

Image Search Results


Figure 1. Disruption of apical-basal polarity by dominant-negative CDH1 mutants in the Matrigel overlay culture system or by Cdc42 knockout in the developing mouse pancreas alters beta cell specification (A) Design of dominant-negative CDH1 mutants with deletions in the extracellular domain (CDH1DE) or the p120ctn-binding site (CDH1DP). mCh, internal ribosome entry site (IRES)-coupled mCh (red fluorescent protein) reporter. (B) Confocal images of S5 (day 13) differentiated cells (± 72 h Dox treatment). EZR, EZRIN, green; BCAT, beta-catenin, gray; mCh, mCherry, red. Scale bar, 50 mm. (C) Flow cytometry quantification of INS single-positive beta cells and GCG positive alpha cells in mCherry+ and mCherry subpopulations after Dox treatment (S4–S6). CDH1DE and CDH1DP mutations led to the same degree of reduction in the INS+GCG/GCG+ ratio in mCherry+ mutant cells compared with mCherry

Journal: Developmental cell

Article Title: Pancreatic alpha and beta cell fate choice is directed by apical-basal polarity dynamics.

doi: 10.1016/j.devcel.2025.02.008

Figure Lengend Snippet: Figure 1. Disruption of apical-basal polarity by dominant-negative CDH1 mutants in the Matrigel overlay culture system or by Cdc42 knockout in the developing mouse pancreas alters beta cell specification (A) Design of dominant-negative CDH1 mutants with deletions in the extracellular domain (CDH1DE) or the p120ctn-binding site (CDH1DP). mCh, internal ribosome entry site (IRES)-coupled mCh (red fluorescent protein) reporter. (B) Confocal images of S5 (day 13) differentiated cells (± 72 h Dox treatment). EZR, EZRIN, green; BCAT, beta-catenin, gray; mCh, mCherry, red. Scale bar, 50 mm. (C) Flow cytometry quantification of INS single-positive beta cells and GCG positive alpha cells in mCherry+ and mCherry subpopulations after Dox treatment (S4–S6). CDH1DE and CDH1DP mutations led to the same degree of reduction in the INS+GCG/GCG+ ratio in mCherry+ mutant cells compared with mCherry

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Latrunculin B Sigma Aldrich #L5288 Heparin Sigma Aldrich #H3149-100KU Bovine serum albumin (BSA) Proliant #7500804 Bovine serum albumin (BSA) Roche #10775835001 Bovine serum albumin (BSA) Sigma Aldrich #B4287 Y-27632 Merck Millipore #688000 45 % glucose solution in water Sigma Aldrich #G8769 7.5% sodium bicarbonate solution Thermo Fisher #25080094 Forskolin Sigma Aldrich #F6886 3-Isobutyl-1-methylxanthin Thermo Fisher #J64598.MC G418 Thermo Fisher #10131027 Hygromycin Thermo Fisher #10687010 Doxycycline Sigma-Aldrich #D3447 Critical commercial assays RNeasy Mini Kit Qiagen #74106 RNeasy Micro Kit Qiagen #74004 iScript cDNA Synthesis Kit BIO-RAD #1708891 KAPA HiFi HotStart Library Amplification Kit Roche #07958951001 cAMP XP@ Assay Kit Cell Signaling #4339 P3 Primary Cell Kit Lonza #V4XP-3024 LIVE/DEAD Fixable Violet Dead Cell Stain Kit Thermo Fisher #L34964 Deposited data scRNA-seq datasets This paper GSE224286 Experimental models: Cell lines SA121 Takara bio N/A H1 Beydag-Tasöz et al.34 (University of Copenhagen) N/A Experimental models: Organisms/strains Cdc42fl/fl R26-YFP+/+ mice In house N/A Pdx1-Cre+/- Cdc42fl/wt mice In house N/A Oligonucleotides siRNA against EGR1 Thermo Fisher #S4537; #S4538 Negative control siRNA Thermo Fisher #4390843 Recombinant DNA Mutant CDH1 sequences Addgene #45770; #45773 PiggyBac destination vector PB-TAC-ERN Addgene #80475 Cas9 expression vector (pX458-HF1) Addgene #48138 Software and algorithms Adobe Photoshop 2022 Adobe https://www.adobe.com Adobe Illustrator 2023 Adobe https://www.adobe.com Imaris 8.4, 9.0 Oxford Instruments https://imaris.oxinst.com/ Fiji 2.0/ImageJ NIH Image http://imagej.nih.gov/ij GraphPad Prism 10 GraphPad https://www.graphpad.com FlowJo 10 BD https://www.flowjo.com FCS Express 7 De Novo Software https://denovosoftware.com ZEN (blue edition) ZEISS https://www.micro-shop.zeiss.com Developmental Cell 60, 1–13.e1–e5, July 7, 2025 e2

Techniques: Disruption, Dominant Negative Mutation, Knock-Out, Binding Assay, Flow Cytometry, Mutagenesis